Isotype : IgM,
Origin, source : Mice were immunised with a ynthetic peptide (20µg/doses) coupled to KLH ( Keyhole limpet hemacyanin). The peptide of 14 amino acids (RPLHSYRQQLELPC) is located in the C-Terminal part of the MUC6 protein. The fusion was performed with the P3 myeloma cell line.
Cross-reactivity : On going experiments.
Binding Assays : The EU-MUC6a recognises specifically the MUC6 gastric mucin.
Immunohistochemistry:
Results of immunohistochemistry experiments
performed in Carme De Bolos's lab :
The expriments were performed on parrafin embedded
sections.
Reductiobn was not used but antigen retrival
(0.01M Sodium Citrate pH:6; 5-10 min; 100ºC ) was perfomred.
Inhibition assays: The EU_MUC6a reactivity was inhibited by MUC6 gastric mucin.
Positive Tissues | Negative Tissues |
Stomach 16/16
Duodenum 1/2 Colon 5/5 Gallbladder 2/4 Ovary 1/2
|
Salivary gland 0/2
Esophagus 0/2 Jejunum 0/3 Ileum 0/2 Pancreas 0/4 Liver 0/2 Bronchial epith. 0/5 Kidney 0/4 Bladder 0/2 Endocervix 0/3 Exocervix 0/4 Endometrium 0/3 Parathyroid gland 0/2 |