Isotype : IgG1, K light chain
Origin, source : Female BALB/C, 6 week old mice were immunised with a synthetic peptide (Lum7-1) coupled to KLH ( Keyhole limpet hemacyanin). The peptide of 17amino acids ( E G R E R D H E L R H R R H H H Q ) is located in the N-terminal part of the MUC7 protein whitin the Histatin-like domain. The parental myeloma cell line was sp2/0. The hybridomas were creened on ELISA assay against a purofied peparation of MUC7.
Cross-reactivity : On ELISA assay the EU-MUC7a antibody does not cross-react with other mucins (MUC2 red-unred, MUC5AC, MUC6, MUC5B, MUC4 N-terminal recombinant protein) or with blood-group substances (substances A, H, Lea, Leb from ovarian cyst fluid).
Binding Assays : The EU-MUC7a antibody was tested on Elisa Assay with a purified preparation of MUC7 and with fresh saliva. The antibody showed strong reactivity with the purified preparation and with the saliva.
Immunoblotting: EU-MUC7a recognizes specifically the MUC7 protein in Saliva.
Immunohistochemistry:
The results of immunohistology show that the antibody stains the tissues
as expected and do not show non-specific staining in other tissues.