Literature references :
Reis CA, David L, Carvalho F, Mandel U, de Bolos C, Mirgorodskaya
E, Clausen H, Sobrinho-Simoes M. Immunohistochemical study of
the expression of MUC6 mucin and co-expression of other secreted mucins
(MUC5AC and MUC2) in human gastric carcinomas.
J Histochem Cytochem. 2000 Mar;48(3):377-88.
Isotype : IgG1
Origin, source : Mice were immunised with a synthetic peptide (SFQTTTTYPTPSHPQTTLPC) conjugated with KLH correspoding to a part of the MUC6 tandem repeat. The fusion was perfomed with the NS-1 myeloma cells.The mouse sera was tested by ELISA, immunohistochemistry and Western blotting.
Specificity : The CLH5 monoclonal antibody reacts with the peptide and recognises mucin extracted from gastric mucosa on Western blotting. The antibody shows a stronger reactivity after deglycosylation.
Cross-reactivity : CLH5 shows no cross-reactivity with other mucin peptides on ELISA.
Immunoblotting : The CLH5 antibody gives a positive signal with mucin extracted from gastric mucosa, the signal is stronger after deglycosylation.
Immunohistochemistry, results from the publication:
On fixed section.
| Positive normal tissues | Negative normal tissues |
| Stomach Antrum (pyloric glands, cytoplasmic
staining)
Stomach Body (Mucous cells of the neck zone, cytoplasmic staining) Gallbladder (Focal staining of deep glands, cytoplasmic staining) Pancreas (Few acinar cells, cytoplasmic staining) Duodenum (Brunner glands, cytoplasmic staining) |
Colon
Ileum Jejunum Liver Breast Bronchus Endocervix Kidney Ovary Prostate Skin Spleen Thyroid |
Results of immunohistochemistry and experiments performed in Jacques Bara's lab
:
| Tissues | Immunohistology | ISH | |
| Oesophageal glands | - | ND | |
| Cardia | Surface
Glands |
-
+++ |
ND
ND |
| Fundus | Surface
Glands |
-
+++ |
-
+++ |
| Pylorus | Surface
Glands |
-
+++ |
-
+++ |
| Duodenum | Villi
Brunner's glands |
-
+++ |
ND
ND |
| Jejunum | Villi
Glands |
-
- |
-
- |
| Ileum | Villi
Glands |
ND
ND |
++ |
| Colon | - | - | |
| Prostate | utriculus | - | ??? |
| Conjunctiva | - | - | |
| Endocervix | - | ++ | |
| Respiratory tract | Surface
Glands mucus cells Serous cells |
-
- - |
-
- - |
| Bile duct | - | + | |
| Pancreas | Islets of Langherans | - | - |
| Pancreas | Wirsung duct
Small ducts |
+++
- |
ND
- |
| Gall Bladder | ++ | +++ |
Other publications using the antibody :
Carvalho F, David L, Aubert JP, Lopez-Ferrer
A, De Bolos C, Reis CA, Gartner F, Peixoto A, Alves P, Sobrinho-Simoes
M. Mucins and mucin-associated carbohydrate antigens expression
in gastric carcinoma cell lines.
Virchows Arch 1999 Nov;435(5):479-85
Machado JC, Nogueira AM, Carneiro F, Reis CA,
Sobrinho-Simoes M. Gastric carcinoma exhibits distinct types
of cell differentiation: an immunohistochemical study of trefoil
peptides (TFF1 and TFF2) and mucins (MUC1, MUC2, MUC5AC, and MUC6).
J Pathol. 2000 Mar;190(4):437-43.
Reis CA, David L, Correa P, Carneiro F, de
Bolos C, Garcia E, Mandel U, Clausen H, Sobrinho-SimoesM. Intestinal
metaplasia of human stomach displays distinct patterns of mucin (MUC1,
MUC2, MUC5AC, and MUC6) expression.
Cancer Res. 1999 Mar 1;59(5):1003-7.
Nogueira AM, Machado JC, Carneiro F, Reis CA,
Gott P, Sobrinho-Simoes M. Patterns of expression of trefoil
peptides and mucins in gastric polyps with and without malignant
transformation.
J Pathol. 1999 Apr;187(5):541-8.
Note : Made in H. Clausen
Laboratory.