Literature references :
Gambus G, de Bolos C, Andreu D, Franci C, Egea G, Real FX.
Detection of the MUC2 apomucin tandem repeat with a mouse monoclonal antibody.
Gastroenterology 1993 Jan;104(1):93-102
Isotype : IgM
Origin, source : Mice were immunised with the deglycosylated mucin isolated from LS174T cell line. The parental cell were NS-1 mouse myeloma cells. Hybridomas were screened by indirect immunofluorescence on cultured LS174T cells, SK-MEL-131 melanoma cells and colon cancer tissues.
Specificity : The LDQ10 antibody reacts with the deglycosylated HT-29 MTX colon cancer mucin.
Cross-reactivity : The LDQ10 antibody does not react with peptides from MUC1, MUC3, and MUC4 tandem repeat.
Binding Assays : Strong reactivity with a peptide located in the C-terminal part of the MUC2 tandem repeat (VTPTPTPTGTQTC). Positive signal was obtained with the deglycosylated HT-29 MTX mucin but not with the native mucin.
Immunoblotting : Positive signal with HT-29, HT-29 MTX lysates and WIDR-FBS cells.
Results of immunohistochemistry experiments performed on Fresh Frozen tissues from the reference paper:
Normal human tissues:
| Positive | Negative |
| Colon (epithelium)
Duodenum (epithelium) Jejunum (epithelium) Ileum (epithelium) Stomach (epithelium) Pancreas (Acini, ducts) Kidney (Proximal tubules, Bowman's capsule) Cervix (Exocervix) |
Colon (Peyer's patch and connective tissue)
Esophagus (Epithelium) Salivary gland (Epithelium) Breast (Acini and ducts) Lung (Bronchial epithelium and pneumocytes) Pancreas (Islets) Kidney (Glomerulus, distal tubulus,collecting tubes) Cervix (Endocervix) Uterus (Myometrium and endometrium) Skin (Epidermis, sweat gland and melanocytes) Thyroid (Epithelium) Spleen (White pulp and red pulp) Lymph node |
Human cancer tissues
| Positive | Negative |
| Colorectal (19/27) Stomach (3/4)
Ovary Breast (7/10) Pancreas (6/11) |
Bladder (6/8)
Esophagus Larynx Vulva Lung Melanoma Kidney Sarcoma leukemia/lymphoma |
Results of immunohistochemistry and experiments performed in Jacques Bara's lab:
| Tissues | Immunohistology | ISH | |
| Oesophageal glands | - | ND | |
| Cardia | Surface
Glands |
+++
- |
ND
ND |
| Fundus | Surface
Glands |
-
+ |
-
- |
| Pylorus | Surface
Glands |
-
- |
-
+ |
| Duodenum | Villi
Brunner's glands |
+++
- |
ND
ND |
| Jejunum | Villi
Glands |
+++
+++ |
+++
+++ |
| Ileum | Villi
Glands |
+++
+++ |
+++
+++ |
| Colon | +++ | +++ | |
| Prostate | utriculus | - | ?? |
| Conjunctiva | - | +/-* | |
| Endocervix | - | +* | |
| Respiratory tract
|
Surface
Glands mucus cells Serous cells |
+++/-
- - |
++
- - |
| Pancreas | Islets of Langherans | - | - |
| Pancreas | Wirsung duct
Small ducts |
+++
- |
ND
- |
| Gall Bladder | ++/- | ++ |
* RW McKenzie et al. Invest.Ophtalmo. Vis. Sci. 41,703-708 (2000)
Other publications using the antibody
:
Mian C, Pycha A, Wiener H, Haitel A, Lodde M, Marberger M. Immunocyt:
a new tool for detecting transitional cell cancer of the urinary tract.
J Urol 1999 May;161(5):1486-9
Note: Made
in F.X. Real laboratory