Literature references :
Hovenberg HW, Davies JR, Herrmann A, Linden CJ, Carlstedt I.
MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions.
Glycoconj J 1996 Oct;13:839-47
Origin, source : Rabbits were immunised with a synthetic peptide coupled to KLH (Keyhole limpet hemacyanin). The peptide of 14 amino acid (NGLQPVRVEDPDGC) is located in the carboxy-terminal region of the MUC2 protein.
Specificity : The Lum2-3 antibody recognizes the MUC2 glycoprotein only after reduction.
Cross-reactivity : Lum2-3 does not react with the mucins MUC5B, (either unreduced or reduced), Muc5AC or Muc6 on ELISA plates. No cross reactivity has been observed with blood group substances ( A, LEA, LEB, H) obtained from ovarian cyst fluid.
Binding Assays : The antiserum was tested on ELISA Assay against colonic mucin from isopycnic and rate-zone gradients and from ion-exchange HPLC Lum2-3 recognises the insuloble subunit from human colon.
Results of immunohistochemistry experiments
performed in Carme De Bolos's lab :
On frozen tissues fixed in formalin
Treatment : pressure cooker in citrate buffer
| Positive tissues | Negative tissues |
| Colon (5/5)
Stomach (4/6) Duodenum (2/2) Jejunum (3/3) Ileum (2/2) |
Salivary gland (0/2)
Esophagus(0/2) Anal mucosa (0/1) Pancreas (0/4) Liver (0/3) Kidney (0/3) Bladder (0/1) Breast (0/1) Ovary (0/3) Exocervix (0/5) Endocervix (0/4) Fallopian tube (0/1) Testis (0/3) Epydidimun (0/2) Defferent duct (0/2) Seminal vesicle (0/1) Prostate (0/1) Thyroid gland (0/2) Parathyroid gland (0/3) Bronchial epithelium (0/3) |
Results of immunohistochemistry experiments
performed in Jean-Pierre Aubert's lab :
Fixation in neutral buffered formalin
treatment: pressuse cooker in citrate buffer
| Positive tissues |
| Bronchial epithelium (surface epitheluium +, serous glands +) |
| Small intestine (goblet cells +++) |
| Gall bladder (surface epithelium ++, crypts ++) |
| HT29 cells in culture ( 20% +) |
Results of immunohistochemistry and experiments performed in Jacques Bara's Lab
:
| Tissues | Immunohistology | ISH | |
| Oesophageal glands | - | ND | |
| Cardia | Surface
Glands |
+/-
- |
ND
ND |
| Fundus | Surface
Glands |
+/-
- |
-
- |
| Pylorus | Surface
Glands |
+/-
- |
-
+ |
| Duodenum | Villi
Brunner's glands |
+++
- |
ND
ND |
| Jejunum | Villi
Glands |
+++
+++ |
+++
+++ |
| Ileum | Villi
Glands |
+++
+++ |
+++
+++ |
| Colon | +++- | +++ | |
| Prostate | (utriculus) | ++++ | ?? |
| Conjunctiva | - | +/-* | |
| Endocervix | + | +* | |
| Respiratory tract
|
Surface
Glands mucus cells Serous cells |
+++/-
- - |
++
- - |
| Bile duct | -/+++ | - | |
| Pancreas | Islets of Langherans | - | - |
| Pancreas | Wirsung duct
Small ducts |
+++
- |
ND
- |
| Gall Bladder | - | ++ |
* RW Mc Kenzie et al. Invest.Ophtalmo. Vis. Sci. 41,703-708 (2000)
Other publications using the antibody :
Herrmann A, Davies JR, Lindell G, Martensson S, Packer NH, Swallow
DM, Carlstedt I. Studies on the "insoluble" glycoprotein complex
from human colon. Identification of reduction-insensitive MUC2 oligomers
and C-terminal cleavage.
J Biol Chem. 1999 May 28;274(22):15828-36.
Davies JR, Svitacheva N, Lannefors L, Kornfalt R, Carlstedt I. Identification
of MUC5B, MUC5AC and small amounts of MUC2 mucins in cystic fibrosis airway
secretions.
Biochem J. 1999 Dec 1;344 Pt 2:321-30.
Note : Made for the EU Consortium