Literature reference (previous batch) :
Hovenberg HW, Davies JR, Carlstedt I.  Different mucins are produced by the surface epithelium and the submucosa in human trachea: identification of MUC5AC as a major mucin from the goblet cells.
Biochem J. 1996 Aug 15;318 ( Pt 1):319-24

Origin, source : Rabbits were immunised with a synthetic peptide coupled with a carrier, the KLH ( Keyhole limpet hemacyanin). The peptide of 12 amino acid (RNQDQQGPFKMC) is repeated at least twice in cysteine rich domains of the tandem repeat region of the MUC5AC mucin.

Specificity :The Lum5-1 polyclonal antibody reacts with the respiratory tract mucins extracted from chronic sputum and normal secretions.

Cross-reactivity : Lum5-1 does not react with the mucin MUC2, (either unreduced or reduced), but in London it was found to give a slightly positive signal with pooled fractions of mucin MUC5B (either unreduced or reduced), and MUC6 on ELISA plates.  No cross reactivity was observed with blood group substances ( A, LEA, LEB, H) obtained from ovarian cyst fluid.

Binding Assays : The antiserum was tested on ELISA against respiratory tract mucins extracted from chronic sputum and normal secretions.

Immunoblotting : Lum5-1 antibody recognises the gel fraction (reduced or unreduced) and the soluble fraction (reduced) of induced sputum.

Results of immunohistochemistry from the reference paper :
 

Positive tissues:	Negative tissues:
Stomach (surface epithelium) Duodenum (some rare goblet cell near pylorus duodenum junction) Bronchial epithelium (goblet cell and some rare mucus cell in the glands (faintly)) Gallbladder (faintly) Endocervix  Oviduct (mucus cells) Prostate (mucus cells in the prostatic utriculus) Conjunctiva (Goblet cells)	Oesophagus Pancreas  Brünner's glands Duodenum Jejunum Ileum Colon Liver Lung  Kidney  Ovary Bladder  Endometrium Adrenal glands

Results of immunohistochemistry experiments performed in Carme De Bolos's lab :
 

Positive tissues	Negative tissues
Stomach (8/8) Duodenum (2/2) Jejunum (3/3) Ileum (2/2) Colon (2/5)  Bronchial epithelium (2/2) Gallbladder (1/1) Breast (2/3) Endocervix (4/4) Ovary (2/2)	Salivary gland (0/2) Oesophagus(0/2) Pancreas (0/4) Liver (0/3) Kidney (0/3) Bladder (0/1) Thyroid gland (0/2) Parathyroid gland (0/3) Exocervix (0/4) Epydidimis (0/2) Defferent duct (0/2) Prostate (0/1) Fallopian tube (0/1) Testis (0/3) Anal mucosa(0/1) Seminal vesicle (0/1)

Results of immunohistochemistry and in situ hybridization experiments performed in Jacques Bara's lab :
The Lum5-1 antibody works on ethanol fixed tissues and shows the same pattern as anti-M1 MAbs (21M1 and 45M1) and all the other anti-M1 MAbs (1-13M1, 2-11M1, 2-12M1,9-13M1, 58M1 see http://www.rangueil.inserm.fr/mucines/Antibody.htm
 
 

Tissues		Immunohistology	ISH
Oesophageal glands		-	ND
Cardia	Surface Glands	+++ -	ND ND
Fundus	Surface Glands	+++ -	+++ -
Pylorus	Surface Glands	+++ -	+++ -
Duodenum	Villi Brunner's glands	- -	- -
Jejunum	Villi Glands	- -	- -
Ileum	Villi Glands	- -	- -
Colon		-	-
Prostate	utriculus	+	???
Conjunctiva		++++	++++
Endocervix		+++	++
Respiratory tract	Surface Glands mucus cells Serous cells	++++ -/++ -	+++ +++/-* -
Bile duct		-	-
Pancreas	Islets of Langherans	-	-
Pancreas	Wirsung duct Small ducts	- -	ND -
Gall Bladder		+	+

* Carme De Bolos results

Other literature references (using previous batch the antibody) :
Hovenberg HW, Davies JR, Carlstedt I.  Different mucins are produced by the surface epithelium and the submucosa in human trachea:  identification of MUC5AC as a major mucin from the goblet cells.
Biochem J. 1996 Aug 15;318 ( Pt 1):319-24.
 

Note : Made for the EU Consortium