Origin, source : Rabbits were immunised with a synthetized peptide coupled with a carrier, the KLH ( Keyhole Limpet Hemacyanin). The peptide of 14 amino acid (RPLHSYEQQLELPC) is located in the carboxy terminal region and precisely in the CK domain of the MUC6 mucin.

Cross-reactivity : No cross reactivity was observed with blood group substances ( A, LeA) obtained from ovarian cyst fluid.

Binding Assays : The Lum6-3 antibody recognizes the purified protein on ELISA assays.

Immunohistochemistry:

Results of immunohistochemistry experiments performed in Carme De Bolos's lab :
Treatment: Reduction
 

Positive tissues	Negative tissues
Stomach (9/9) Duodenum (1/1) Jejunum (3/3) Anal mucosa (1/1) Gallbladder (2/2) Endocervix (1/7) Exocervix (1/7)	Salivary gland (0/2) Oesophagus (0/1) Ileum (0/3) Colon (0/3) Pancreas (0/2) Liver (0/3) Bronchial epithelium (0/3) Kidney (0/3) Bladder (0/1) Breast (0/3) Ovary (0/1) Fallopian tube (0/1) Testis (0/1) Epydidimis (0/1) Vas deferens  (0/3) Seminal vesicle (0/1) Prostate (0/2) Thyroid gland (0/2) Parathyroid gland (0/2)

Results of immunohistochemistry and in situ hybridization experiments performed in Jacques Bara's lab :
 Ethanol fixed sections after mercaptoethanol reduction:
 

Tissues		Immunohistology	ISH
Oesophageal glands		-	ND
Cardia	Surface Glands	- +++	ND ND
Fundus	Surface Glands	- +++	- +++
Pylorus	Surface Glands	- +++	- +++
Duodenum	Villi Brunner's glands	- +++	ND ND
Jejunum	Villi Glands	- -	- -
Ileum	Villi Glands	- -	++
Colon		-	-
Prostate	(utriculus)	-	???
Conjunctiva		-	-
Endocervix		+	++
Respiratory tract	Surface Glands mucus cells Serous cells	-* -* +*	- - -
Bile duct		-	+
Pancreas	Islets of Langherans	ND	-
Pancreas	Wirsung duct Small ducts	ND ND	ND -
Gall Bladder		+/-	+++

* Microwave and DTT treatment

More results from Jacques Bara's lab:
 

Positive tissues:	Negative tissues:
Stomach  Oviduct (mucus cells, faintly) Adrenal glands (Zona fasciculata)	Lung Kidney (0/3) Bladder (0/1) Ovary (0/1) Epydidimis Seminal vesicle

Note : Made for the EU Consortium