Literature references :
Nielsen PA, Mandel U, Therkildsen MH, Clausen H.   Differential expression of human high-molecular-weight salivary mucin (MG1) and low-molecular-weight salivary mucin (MG2).
J Dent Res 1996 Nov;75(11):1820-6

Isotype : IgG1

Origin, source : Mice were immunised with a synthesized peptide coupled with a carrier, the KLH (Keyhole limpet hemacyanin). The peptide of 21 amino acids (CRPKLPPSPNKPPKFPNPHQP) is located in the N-terminal part of the Muc7 protein. Potential O-glycosylation sites in the peptide region were assessed by in vitro O-glycosylation with partially purified porcine submaxillary gland UDP-GalNac. Clones were tested by ELISA against the peptide, by Western blotting and by immunohistochemistry.

Specificity : PANH3 recognises purified MG2 and partially deglycosylated MG2 salivary mucin.

Cross-reactivity : The PANH3 antibody does not show reactivity with other synthetic peptides.

Binding Assays :  The hybridoma clones were tested against the peptide.

Immunoblotting : The PANH3 monoclonal antibody works on Western blot of whole human saliva, glycosylated, partially deglycosylated and fully deglycosylated.

Immunohistochemistry from the reference publication:
On frozen tissues
 

Positive reactivity	Negative reactivity
Serous cells (staining in cytoplasmin granular) in the submandubular, sublingual and small salivary gland of the lip and palateand in the palatine gland.	Mucous cells in the submandibular, sublingual and small salivary glans of the lip and palate Serous cells parotic gland Duct cells Intercalated ducts, striated ducts and excretory ducts.

Note : Made in the H.Clausen Laboratory