Flow Cytometry

Flow Cytometry Core Facility at UCL GOS Institute of Child Health provides state-of-the-art instrumentation and expertise in cell analysis and cell sorting.

Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.

Book an instrument or REGISTER

Only PPMS registered users have access to instruments and analysis computers. The facility has two types of insruments and two free of charge analysis computers. The instruments are either cell analysers or cell sorters. Users can directly book any cell analyser they trained on. Cell sorters are only operated and booked by facility's staff. Users can check the availibility of the cell sorters in the link below and call or send an email to facility's staff indicating the time they would like to book their sort.

Check availability of a CELL SORTER*

*must login with flowcytometryich@googlemail.com account - requires PW that is available at the facility. You can only see availability.

BenchSci: Search published antibody data

Make sure to check that the antibody was validated to be used in flow cytometry (FCM).

FluoroFinder: build your FCM panels

Services Available

cell sorting
cloning: single cell sorting
data acquisition, analysis and interpretation
user training
advice on sample preparation
user assistance and machine troubleshooting
help with design of experiments
sourcing of reagents and techniques; finding alternative approaches
help with data preparation for presentation and publication

We are also open to collaboration.

Funding and Acknowledgements

Please acknowledge use of the facility in all publications. Suggested text: Flow cytometry was performed at the UCL Great Ormond Street Institute of Child Health, supported by the Great Ormond Street Children's Charity (GOSHCC), seed grant reference U09822 (October 2007), UCL Capital Equipment Funding, School of Life and Medical Sciences (September 2012; February 2022).

All research at Great Ormond Street Hospital NHS Foundation Trust and UCL Great Ormond Street Institute of Child Health is made possible by the NIHR Great Ormond Street Hospital Biomedical Research Centre. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health.