The HCBL houses the Opera Phenix and Opera Phenix Plus, two confocal high-content microscopes. These user-friendly microscopes allow for completely automated imaging of cells or tissue, permitting users to acquire thousands of images in a very short amount of time. This makes it possible to test several conditions at the same time and/or analyse multiple phenotypes concurrently. The Opera Phenix is equipped with a robotic arm, which can automatically feed the Phenix with assay plates, allowing for further speed when acquiring images. The latest Opera model, the Opera Phenix Plus comes with an injector module and provides a high frame rate, which makes it ideal to study fast-response events in the cell like calcium flux or membrane potential changes. 

Perkin Elmer Opera Phenix
The Opera Phenix is a confocal spinning disc microscope that has the capability to automatically acquire images in multiwell plates and slides. The automated acquisition advances the imaging process and allows the researcher to study their project in an impartial way. Our users image a wide variety of samples on the Phenix, e.g. single layer cell lines, multilayer neuronal cultures, tissue sections and organoids. The Phenix is equipped with CO2 and temperature control features, enabling the capture of live cell images and time courses. A plate handler and incubator allow the automated imaging of multiple plates.
Specifications:
Objectives: 5x air, 10x air, 20x air, 40x air, 20x water, 40x water, 63x water
Lasers: 375, 425, 488, 561 and 640nm
Emission Filters: 435-515nm, 435-550nm, 435-480nm, 500-550nm, 570-630nm, 650-760nm
Environmental chamber (CO2/Temperature) for live cell imaging
Plate handler and incubator
Harmony 4.9 Software
PresiScan software
PhenoLOGIC machine learning algorithm
Columbus server for image analysis
 
Perkin Elmer Opera Phenix Plus
The Phenix Plus shares all the features of the Opera Phenix but has a higher imaging frame rate and is equipped with an injector module for the addition of reagents and compounds while capturing images. As a result, it is well-suited for observing rapid-response assays.
Specifications:
Objectives: 5x air, 10x air, 20x air, 40x air, 20x water, 40x water, 63x water
Lasers: 405, 488, 561 and 640nm
Emission Filters: 435-480nm, 435-515nm, 435-550nm, 435-480nm, 465-480nm, 465-530nm, 500-530nm, 500-550nm, 515-550nm, 570-630nm, 571-596nm, 605-630nm, 650-680nm, 650-680nm, 650-760nm, 690-720nm
Injector module
Environmental chamber (CO2/Temperature/Humidity) for live cell imaging
Fast 100 fps frame rate
Harmony 5.1 software
PresiScan software
PhenoLOGIC machine learning algorithm
Columbus server for image analysis
 
Columbus analysis software
Columbus is an image analysis software ideal for the display and analysis of large image sets. It comes with preprogrammed building blocks for a user-friendly analysis experience. We host it on an LMCB server, and it is accessible via web interface from across UCL.