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last updated November 2023
Request
transgenic mice from the Bill Richardson/ Nicoletta Kessaris
labs
We thank the UK Medical Research
Council, The Wellcome Trust, the European Research Council,
the Biotechnology and
Biological Sciences Research Council, the US National Institutes of Health
and The Royal Society
for past and present support.
We have generated a number of transgenic strains,
mainly BAC or PAC transgenics
by pronuclear injection. If you wish to obtain
these please follow the steps below.
1. email Bill Richardson
(w.richardson@ucl.ac.uk)
and/or Nicoletta Kessaris
(n.kessaris@ucl.ac.uk) outlining your proposed project in a
few sentences.
2. Some popular lines [e.g. Pdgfra-CreER(T2), Fgfr3-iCreER(T2), Sox10-iCreER(T2)] are
listed on the UCL Business website https://xip.uclb.com
->
Biological Materials, from where you can download and complete
an MTA, as well as obtain further information such as
genotyping protocols. Please check xip.uclb.com in any
case because one or two lines are listed there that are NOT listed in
the table below.
3. Sometimes the line you want will be available closer to you
from a third party lab - we can provide contact details.
4. For health status of the mice please contact Matthew Grist (m.grist@ucl.ac.uk) in the first instance.
We normally ship 2 or 3 males of breeding age. We regret that
we cannot ship embryos or sperm.
Please note that we depend on you to pay costs. Shipment
is organized by Gabi Sturges (gabrielle.turner@ucl.ac.uk), manager of our Biological
Services Unit. Please contact our lab manager Matthew Grist
(m.grist@ucl.ac.uk) first.
The
following mice are available, as well as some others not
listed here:
Strain
|
Animal
code
|
Contact
|
Vector
|
Made
by
|
References
|
MTA
|
Genotyping protocol
|
Comments/
expression
pattern
|
Dbx1-iCre
|
H142
|
Kessaris cc Richardson
|
PAC
|
Fogarty/Kessaris
|
1,
4,
5
|
Dbx1-iCre
|
Dbx1-iCre
or iCre
|
Cortico-striatal boundary,
spinal cord p1, p2, dP6, dP5
|
Dlx1-lox-Venus-lox
|
A666
|
Kessaris
|
BAC
|
Rubin/Alfonsi/
Kessaris
|
16
(suppl info)
|
Dlx1-Venus
|
Dlx-Venus
|
Forebrain
|
Emx1-iCre
|
H151
|
Kessaris cc Richardson
|
PAC
|
Fogarty/Kessaris
|
3,
(suppl info),
4,
5
|
Emx1-iCre
|
Emx1-iCre
or iCre
or use UV lamp at P0-P1*
|
Cortical
neuroepithelium
|
Emx1-CreERT2
|
G199
|
Kessaris cc Richardson
|
PAC
|
Fogarty/Kessaris
|
3,
5
|
Emx1-CreERT2
|
Emx1-CreERT2
|
Cortical
neuroepithelium
|
Fgfr3-iCreERT2
|
K121
|
Kessaris cc Richardson
|
PAC
|
Mitsumori/Kessaris
|
7 (suppl info),
13
(suppl info 1,
2)
|
Fgfr3-iCreERT2
|
iCre
|
Neuroepithelial cells, SVZ cells,
protoplasmic and fibrous astrocytes
|
Gsh2-iCre
|
H153
|
Kessaris cc Richardson
|
PAC
|
Kessaris
|
3,
(suppl info),
4,
5,
10,
12
|
Gsh2-iCre
|
Gsh2-iCre
or iCre
|
LGE/MGE/CGE
|
Lhx6-iCre
|
N104
|
Kessaris
|
BAC
|
Kessaris
|
4,
6,
16
(suppl info),
17
|
Lhx6-iCre
|
iCre
or use UV lamp at early stages*
|
MGE
|
Msx3-iCre
|
K133
|
Kessaris cc Richardson
|
PAC
|
Fogarty/Kessaris
|
Unpublished
|
Msx3-iCre
|
Msx3-iCre
or iCre
or use UV lamp at early stages*
|
Dorsal
spinal cord dP1, dP2, dP3, dP4 and neural crest
|
Nkx2.2-CreERT2
|
H101
|
Kessaris cc Richardson
|
PAC
|
Taveira-Marques/Kessaris
|
Unpublished
|
Nkx2.2-CreERT2
|
Nkx2.2-CreERT2
|
Ventral
spinal cord p3
|
Nkx2.1-iCre
|
G178
|
Kessaris cc Richardson
|
PAC
|
Kessaris
|
3,
(suppl.
info), 4,
10,
12,
16
(suppl info),
17
|
Nkx2.1-iCre
|
Nkx2.1-iCre
or iCre
|
MGE
|
Nkx2.1-iCre-ERT2
|
K185
|
Kessaris cc Richardson
|
PAC
|
Kessaris
|
Unpublished
|
Nkx2.1-iCre-ERT2
|
Nkx2.1-iCre
or iCre
|
MGE
|
Nkx6.2-iCre
|
L129
|
Kessaris
|
BAC
|
Kessaris
|
4
|
Nkx6.2-iCre
|
iCre
|
LGE/MGE
boundary, spinal cord
|
Olig2-Cre
|
E193
|
Richardson
|
Knock-in
|
Rowitch/Kessaris
|
3,
(suppl.
info),
14
|
Olig2-Cre
|
Olig2-Cre
|
Spinal cord
pMN, oligodendrocyte lineage
|
Olig2-NLS-eGFP
|
H152
|
Richardson
|
PAC
|
Callard/Kessaris
|
Unpublished
|
Olig2-NLS-eGFP
|
Olig2-GFP
|
Spinal cord
pMN, oligodendrocyte lineage
|
Pdgfra-CreERT2
|
H126
|
Richardson
|
PAC
|
Jamen/Kessaris
|
7 (suppl info),
15
|
Pdgfra-CreERT2
|
Pdgfra-CreERT2
|
Oligodendrocyte
progenitors postnatally (ectopic dorsal
expression in embryo)
|
Sox1-iCreERT2
|
K184
|
Kessaris cc Richardson
|
BAC
|
Kessaris
|
Unpublished
|
Sox1-iCreERT2
|
iCre
|
Embryonic
neuroepithelium and
adult neural stem cells
|
Sox10-Cre
|
F155
|
Kessaris cc Richardson
|
PAC
|
Iannarelli/Kessaris
|
2,
8,
9,
11
|
Sox10-Cre
|
Sox10-Cre
or use UV lamp at early stages*
|
Oligodendrocyte lineage, neural
crest, Schwann cells
|
Sox10-
iCreERT2 |
N388
|
Richardson
|
PAC
|
Paes de Faria/Li
|
18, (suppl info) |
Sox10-
iCreERT2 |
Sox10-
iCreERT2
|
as above |
Sox10-lox-GFP-STOP-lox-DTA
|
K152
|
Kessaris cc Richardson
|
PAC
|
Iannarelli/Kessaris
|
3,
(suppl.
info)
|
Sox10- GFP-DTA
|
Sox10-DTA or use
UV lamp at early stages
|
Oligodendrocyte lineage, neural
crest, Schwann cells
|
Zic4-iCre
|
A611
|
Kessaris
|
BAC
|
Kessaris/Rubin
|
16
(suppl info)
|
Zic4-iCre
|
iCre
|
Septal telencephalic
and diencephalic
regions
|
*
A UV lamp can be used to genotype these mice when a Cre reporter allele expressing a
fluorescent protein is also present