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The 2.2 MDa molecular weight of the bacterial 70S ribosome, combined with its limited solubility (≤10 µM), present significant technical challenges to performing high-resolution studies of co-translational folding in ribosome-nascent chain complexes. We are therefore exploring and developing a range of strategies to enhance the sensitivity and resolution of these investigations, from varied isotopic labelling schemes to novel NMR pulse programs, sampling schemes and analysis methods.

Further reading:

• An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR. Waudby CA, Christodoulou J. J Magn Reson (2012) 219, 46-52. pubmed | pdf
• Probing side-chain dynamics of a ribosome-bound nascent chain using methyl NMR spectroscopy. Hsu STD, Fucini P, Cabrita LD, Christodoulou J, Dobson CM. J Am Chem Soc (2009) 131, 8366-7. pubmed | pdf