Cell analysis guidelines

UCL ICH Flow Cytometry Core Facility users have several data analysis programs at their disposition:

• FACSDiva and FCAP software from Becton Dickinson
• Summit, CytExpert and Kaluza software from Beckman Coulter
• FlowJo from TreeStar, for universal Flow Cytometry Standard (FCS) files analysis.

Dedicated Mac and PC computers running FlowJo, Summit, ModFiT, Kaluza and FACSDiva analysis software are available for free to all facility users. The facility also offers FCAP array software for CBA analysis.

With the rapid advances in flow cytometry technology, new fully digitized instruments are replacing the old analog machines and new software are developed. To understand and correctly interpret flow cytometry data, it is becoming essential to know how these data are generated and recorded. There are few papers that explain these important advances and outline guidelines as to how to record and report flow cytometry data.

• Minimum Information about a Flow Cytometry Experiment - ISAC Recommendation
• Optimizing a Multi-colour Immunophenotyping Assay
• Selecting Reagents for Multicolor Flow Cytometry
• Interpreting flow cytometry data: a guide for the perplexed