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The Ear Institute Microscopy Unit is a light and electron microscopy facility.
The Microscopy Unit is providing imaging for staff and students of the Ear Institute, other UCL departments, and external users. We have confocal, multiphoton and widefield light microscopes, and cryogenic and room-temperature scanning and transmission electron microscopy.
Unit Equipment
- Light Microscopy
- Zeiss 880 Inverted Confocal with Airyscan and enviromental chamber
- Zeiss AxioImager.M2 Upright Fluorescence Widefield
- Electron Microscopy
- JEOL 1400Flash TEM with Gatan RIO16 (Room temperature and cryo)
- JEOL 6700F SEM (Room temperature and cryo)
- Sample Preparation
- Sputter/Carbon coater with glow discharge unit (Quorum 1500T)
- Critical Point Dryer (Leica CPD300)
- Freeze Fracture Machine (Balzers 400D)
- High Pressure Freezer (Leica EM ICE)
- Slam Freezer (Leica MM80)
- Nitrogen Slush Freezer (Gatan Alto)
- Freeze Substitution (Leica AFS)
- Ultramicrotomes
- Image Analysis PC
Microscopy Techniques
- Techniques
- Confocal Microscopy
- Widefield Microscopy
- Airyscan
- Live cell imaging
- Correlative light and electron microscopy
- negative stain TEM
- TEM of resin embedded sections
- SEM
- Cryo TEM
- Cryo SEM
- Volume EM
- Electron tomography
- Correlative light and electron microscopy
- Immuno electron microscopy
- Freeze Fracture
Gallery
