Electron Microscopy

The LMCB has been specialising in cell biological EM for over 20 years. The facility comprises state-of-the-art specimen preparation and imaging technologies which, together with two experienced researchers, support the needs of the LMCB groups in all their EM research.  

With experience in a wide range of electron microscopy techniques as well as a broad range of model organisms, we can provide advice, training and practical support for researchers throughout the entire timeline of their projects. For example: planning experiments, sample preparation, imaging and data analysis. The support given is tailored to each project, and can vary from advice and technical support for the more experienced, full training in required techniques and the equipment use for the less experienced researchers or the undertaking of the EM projects ourselves, a collaborative endeavour as part of the ongoing research.

Selected publications

White IJ and Burden JJ (2023). A practical guide to starting SEM array tomography-An accessible volume EM technique. Methods Cell Biol. 2023;177:171-196. Link to PDF.

White IJ, et al (2023), Breaking down the correlative workflow for array tomography: From section collection to image analysis in From 3D light to 3D electron microscopy: Shared experiences from the volume EM community (Science/AAAS, Washington, DC, 2022), p. 6-8

Roubinet C, et al (2021). Asymmetric nuclear division in neural stem cells generates sibling nuclei that differ in size, envelope composition, and chromatin organization. Curr Biol. Sep 27;31(18):3973-3983

Lopes da Silva M, et al (2019). A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport. Dev Cell. 2019 Jun 3;49(5):786-801.e6. doi: 10.1016/j.devcel.2019.04.006.

Russell MR, et al (2017). 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy. J Cell Sci. Jul 21. pii: jcs.188433