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The Rayne facility provides training and access to cutting-edge flow cytometry analysers and cell sorting instruments with both conventional and spectral based technology. UCL staff and students only.
How to get access
Location
Rayne Building, Bloomsbury
Available to
UCL staff and students
Contact
For enquiries, training and registration, contact facility manager: jamie.evans@ucl.ac.uk.
Bookings
Services
The facility offers a range of services including:
- Cell sorting
- Image based cell sorting
- Spectral based cell sorting
- Flow cytometry training
- Spectral flow cytometry analysers
- Conventional flow cytometry analysers
- Panel design / optimisation help and advice
- Experimental design / troubleshooting
- Application and data analysis support.
Researchers can use it for applications such as:
- multicolour immunophenotyping
- fluorescent protein detection (e.g. GFP)
- apoptosis
- cell cycle analysis
- cell viability
- Single cell sorting for genomics and cloning
- FRET
- cytokine detection.
Equipment
The facility is equipped with state-of-the-art flow cytometry analysers / cell sorters with both conventional and spectral based technology. This includes UCL’s only spectral cell sorter with imaging capabilities.
BD FACS Discover S8
The first highspeed full spectral cell sorter capable of sorting on imaging parameters. The cell sorter is contained in a Class 2 biosafety cabinet.
- 5 Laser: 349nm, 405nm, 488nm, 561nm and 637nm
- 78 detectors capable of sorting based on imaging features
- 50+ fluorescent markers.
- Capable of 6-way sorting into 5ml tubes along with collecting into 15ml, 96well, 384 well plates and slides and single cell cloning.
BD FACS Aria (SORP)
This high-speed cell sorter is custom built with higher laser powers for improved sensitivity and resolution over standard Aria-based systems.
- 5 Lasers: 355nm, 405nm, 488nm, 561nm and 640nm
- 20 parameter cell sorting
- Capable of sorting into 5ml/15ml tubes, 96well, 384 well plates and slides and single cell cloning sorting.
Sony ID7000 Spectral Analyser
The first spectral system at UCL with a 320nm laser allowing for greater resolution and sensitivity across the spectrum compared to other 5 laser systems.
- 6 Lasers: 320nm 355nm, 405nm, 488nm, 561nm and 637nm
- 184 detectors capable of 44+ parameter analysis.
- Integrated HTS unit for acquiring from 96well/384wll plates.
BD LSRFortessa X-20 (SORP)
Custom-built with higher laser powers for improved sensitivity and resolution over standard FortessaX20 systems.
- 5 Lasers: 355nm, 405nm, 488nm, 561nm and 640nm
- 20 parameter analysis.
BD LSRFortessa
- 4 Lasers: 405nm, 488nm, 561nm and 640nm
- 17 parameter analysis
- HTS unit for acquiring from 96well and 384 well plate formats.
BDFACS Verse
- 3 Lasers: 405nm, 488nm and 640nm
- 10 parameter analysis
- Accepts 40 tube rack, 96 well plate and 384 well plate formats.
Training
We provide training to help you optimise your experiments and analyse your data effectively. Training is required on the analysers and samples, run independently.
Cell sorting is provided as a service and the systems are run by facility staff.
For training and registration to use the facility, please contact the core facility manager: jamie.evans@ucl.ac.uk.
