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This table shows the microscopes available at the Cruciform and QSH sites, along with their configurations.
More detailed information on all the systems can be found in the tabs to the left.
| Name | Location | Type | Excitation sources | Detectors | Incubator / CO2 | Objective Lenses | Software | Notes |
|---|---|---|---|---|---|---|---|---|
| Zeiss LSM800 Confocal | 2.4 | Inverted, confocal | 405, 488, 561, 640 nm; 7-colour LED | 2x GaAsP PMT, sCMOS camera | Stage top incubator, CO2 | 10x 0.45 Air 20x 0.8 Air 40x 1.3 Oil 63x 1.4 Oil | Zen Blue 2.6 | Correlative Array Tomography (CAT) module |
| Zeiss Axio Scan.Z1 | 2.3 | Slide scanner, widefield | Bright field; HXP 120 lamp | sCMOS camera | No | 5x 0.25 Air 10x 0.45 Air 20x 0.8 Air 40x 0.95 Air | Zen Blue 3.1 | 100 slide automated imaging |
| Zeiss Celldiscoverer 7 with Airyscan 2 confocal | 2.4 | Inverted, widefield, confocal | Bright field; 405, 488, 561, 640 nm; 7-colour LED | sCMOS camera, 2x PMT, Airyscan 2 | Yes + robot automation with separate incubator | 5x 0.35 Air 20x 0.7 Air 20x 0.95 Air 50x 1.2 Water Additional 0.5x /2x magnification for all objectives | Zen Blue 3.5 | Unattended long-term high-resolution imaging |
| IncuCyte S3 | 2.3 TC room | Inverted, widefield | Green / Red LEDs | CMOS camera | Yes | 4x Air 10x Air 20x Air | IncuCyte | Unattended long-term cell monitoring |
| EVOS XL Core | TC rooms | Inverted, widefield | Bright field | None | No | 4x Air 10x Air 20x Air | None | Can be placed in TC hood for colony picking etc |
| Zeiss Axioscope 5 | 1.2 | Upright, widefield | Bright field; 4-colour LEDs | Colour camera | No | 5x 0.16 Air | None | |
| Zeiss Axio Vert.A1 | 2.3 TC room | Inverted, widefield | Bright field; 2-colour LEDs | Monochrome camera | No | 5x 0.15 Air 10x 0.25 Air 20x 0.35 Air 40x 0.55 Air | Zen Blue | |
| Zeiss LSM980 Confocal with Airyscan 2 | 2.3 QSH 413 | Inverted, confocal | 405, 445, 488, 514, 561, 639nm; 4-colour LEDs | 2x PMT, 32x GaAsP array, Airyscan 2 | Yes, CO2 | 2.5x 0.1 Air 10x 0.45 Air 20x 0.8 Air 40x 1.3 Oil 63x 1.4 Oil | Zen Blue 3.5 | 8 Y Airyscan for 8 line parallel Airyscan imaging |
| Leica Stellaris 8 tauSTED / FLIM confocal | 2.3 (office) | Inverted, confocal | 405, 440-790nm White Light Laser, 592 + 775nm STED | 4x Hybrid HyD detectors | Yes, CO2 | 2.5x 0.07 Air 20x 0.75 Air 20x 0.75 Multi-immersion 40x 1.3 Oil 93x 1.3 Glycerol 100x 1.4 Oil | LASX 4 | tauSTED for low power STED of live cells FLIM lifetime imaging |
| Leica Mica Microhub | 2.3 (office) | Inverted, confocal, widefield | LED (Widefield): 365, 470, 555, 625 nm Lasers (confocal): 405, 488, 561, 638 nm | 4x Camera (Widefield) 4x Hybrid HyD detectors (confocal) | Yes, CO2 | 10x 0.32 Air 20x 0.75 Air 63x 1.2 Water 63x 1.4 Oil | LASX 4 | 4 colour simultaneous imaging THUNDER / LIGHTNING computational clearing / deconvolution |
| Cairn Spinning Disk confocal / TIRF / LiveSR | QSH 3 | Inverted, confocal (spinning disk) | LED (confocal): 405, 445, 470, 520, 530, 555, 640 nm Lasers (TIRF): 405, 488, 561, 638 nm | 2x sCMOS camera | Yes, CO2 | 10x 0.45 Air 20x 0.8 Air 40x 1.3 Oil 60x 1.2 Water 60x 1.4 Oil 100x 1.49 Oil | VisiView | 2 camera simultaneous imaging FRAP Photobleaching PALM / STORM super-resolution |
| Perkin Elmer Opera Phenix | 2.3 | Inverted, confocal (spinning disk) | Lasers: 405, 488, 561, 640 nm | 4x sCMOS camera | Yes, CO2 | 5x 0.16 Air 10x 0.3 Air 20x 1.0 Water 40x 1.1 Water 63x 1.15 Water | Harmony | 4 colour simultaneous imaging |
Booking the microscopes and scanners
Most of the above microscopes and scanning systems can be booked using the Infinity system. Contact the microscope manager for more details.