BLAST

We did a BLAST analysis using ncbi protein blast database of all four organisms including the human isoform a as a reference and obtained the below results. The results highlighted in red are the isoforms and species that were previously identified in the FASTA section.

Every homologue got a score depending on how close it is to the reference. The higher the score the more similar it is. E value shows the probability that the score is due to chance; where the lower the E value the less like likely similarity is due to chance.

The coverage shows the length of the sequence that could be aligned compared to the reference.

We have also included a phylogenetic tree that shows the possible evolutionary tree of LAT homologues and how closely they are related.

Query ID: lcl|44204

Description: isoforma

Molecule type: amino acid

Query Length: 262

Graphic Summary:

 

Sequences producing significant alignments:

Accession	Description	Max score	Total score	Query coverage	E value	Max ident	Links
gi|7657293|NP_055202.1	linker for activation of T-cells family member 1 isoform a [Homo sapiens] >gi|14194891|sp|O43561.1|LAT_HUMAN RecName: Full=Linker for activation of T-cells family member 1; AltName: Full=36 kDa phospho-tyrosine adapter protein; Short=pp36; AltName: Full=p36-38 >gi|2828026|gb|AAC39637.1| LAT [Homo sapiens] >gi|119572414|gb|EAW52029.1| linker for activation of T cells, isoform CRA_f [Homo sapiens] >gi|119572415|gb|EAW52030.1| linker for activation of T cells, isoform CRA_f [Homo sapiens]	340	340	90%	1e-114	100%
gi|254939232|CBA11533.1	linker for activation of T-cells family member 1 [Homo sapiens]	334	334	90%	4e-112	99%
gi|254939234|CBA11534.1	linker for activation of T-cells family member 1 [Homo sapiens]	312	312	90%	1e-103	96%
gi|296219835|XP_002756055.1	PREDICTED: linker for activation of T-cells family member 1-like isoform 2 [Callithrix jacchus]	289	289	90%	1e-94	88%
gi|62739155|NP_001014987.1	linker for activation of T-cells family member 1 isoform b [Homo sapiens] >gi|2780223|emb|CAA11218.1| tyrosine-phosphorylated protein of 36 kD (pp36) [Homo sapiens] >gi|2828024|gb|AAC39636.1| LAT [Homo sapiens] >gi|15079451|gb|AAH11563.1| Linker for activation of T cells [Homo sapiens] >gi|119572416|gb|EAW52031.1| linker for activation of T cells, isoform CRA_g [Homo sapiens]	268	268	90%	2e-86	86%
gi|239049118|NP_001014989.2	linker for activation of T-cells family member 1 isoform d [Homo sapiens] >gi|119572412|gb|EAW52027.1| linker for activation of T cells, isoform CRA_d [Homo sapiens]	268	268	90%	5e-86	86%
gi|119572409|EAW52024.1	linker for activation of T cells, isoform CRA_a [Homo sapiens]	264	264	71%	2e-85	100%
gi|332863564|XP_001147360.2	PREDICTED: linker for activation of T-cells family member 1 [Pan troglodytes]	265	265	90%	4e-85	86%
gi|332224752|XP_003261532.1	PREDICTED: linker for activation of T-cells family member 1-like [Nomascus leucogenys]	262	262	90%	3e-84	83%
gi|62739157|NP_001014988.1	linker for activation of T-cells family member 1 isoform c [Homo sapiens] >gi|119572413|gb|EAW52028.1| linker for activation of T cells, isoform CRA_e [Homo sapiens]	261	261	90%	5e-84	86%
gi|355710075|EHH31539.1	hypothetical protein EGK_12631 [Macaca mulatta]	261	261	90%	4e-83	82%
gi|297283724|XP_001102058.2	PREDICTED: linker for activation of T-cells family member 1 [Macaca mulatta] >gi|355756664|gb|EHH60272.1| hypothetical protein EGM_11601 [Macaca fascicularis]	255	255	90%	1e-81	82%
gi|296219833|XP_002756054.1	PREDICTED: linker for activation of T-cells family member 1-like isoform 1 [Callithrix jacchus]	242	242	90%	1e-76	77%
gi|345801822|XP_849910.2	PREDICTED: linker for activation of T-cells family member 1 [Canis lupus familiaris]	186	186	80%	4e-54	64%
gi|311251351|XP_003124570.1	PREDICTED: linker for activation of T-cells family member 1-like [Sus scrofa]	184	184	88%	6e-54	61%
gi|296473278|DAA15393.1	linker for activation of T cells [Bos taurus]	179	179	88%	9e-52	58%
gi|198282101|NP_001098448.1	linker for activation of T-cells family member 1 precursor [Bos taurus] >gi|151554230|gb|AAI49499.1| LAT protein [Bos taurus]	176	176	88%	1e-50	57%
gi|354498022|XP_003511115.1	PREDICTED: linker for activation of T-cells family member 1-like [Cricetulus griseus] >gi|344239494|gb|EGV95597.1| Linker for activation of T-cells family member 1 [Cricetulus griseus]	174	174	90%	4e-50	57%
gi|13540669|NP_110480.1	linker for activation of T-cells family member 1 [Rattus norvegicus] >gi|14194889|sp|O70601.1|LAT_RAT RecName: Full=Linker for activation of T-cells family member 1; AltName: Full=36 kDa phospho-tyrosine adapter protein; Short=pp36; AltName: Full=p36-38 >gi|3021583|emb|CAA04577.1| tyrosine-phosphorylated protein of 36 kD (pp36) [Rattus norvegicus] >gi|149067937|gb|EDM17489.1| linker for activation of T cells, isoform CRA_a [Rattus norvegicus]	166	166	90%	5e-47	53%
gi|301783997|XP_002927386.1	PREDICTED: linker for activation of T-cells family member 1-like [Ailuropoda melanoleuca]	166	166	90%	7e-47	60%
gi|291390878|XP_002711940.1	PREDICTED: linker for activation of T cells-like [Oryctolagus cuniculus]	164	164	90%	3e-46	58%
gi|6754510|NP_034819.1	linker for activation of T-cells family member 1 precursor [Mus musculus] >gi|14194892|sp|O54957.1|LAT_MOUSE RecName: Full=Linker for activation of T-cells family member 1; AltName: Full=36 kDa phospho-tyrosine adapter protein; Short=pp36; AltName: Full=p36-38 >gi|2828028|gb|AAC40054.1| LAT [Mus musculus] >gi|15426469|gb|AAH13337.1| Linker for activation of T cells [Mus musculus] >gi|20429124|emb|CAD27390.1| linker protein [Mus musculus] >gi|74137142|dbj|BAE21976.1| unnamed protein product [Mus musculus] >gi|148685399|gb|EDL17346.1| linker for activation of T cells, isoform CRA_b [Mus musculus]	163	163	90%	7e-46	56%
gi|30354173|AAH52340.1	Linker for activation of T cells [Mus musculus]	161	161	90%	4e-45	56%
gi|26353848|BAC40554.1	unnamed protein product [Mus musculus]	160	160	90%	2e-44	55%
gi|281346516|EFB22100.1	hypothetical protein PANDA_017179 [Ailuropoda melanoleuca]	154	154	87%	1e-42	59%
gi|355699061|AES01004.1	linker for activation of T cells [Mustela putorius furo]	153	153	80%	3e-42	59%
gi|348585016|XP_003478268.1	PREDICTED: linker for activation of T-cells family member 1-like [Cavia porcellus]	149	149	90%	1e-40	56%
gi|254939236|CBA11535.1	linker for activation of T-cells family member 1 [Homo sapiens]	120	120	30%	8e-31	96%
gi|351708926|EHB11845.1	Linker for activation of T-cells family member 1 [Heterocephalus glaber]	125	125	80%	1e-30	50%
gi|56789446|AAH88130.1	Lat protein [Rattus norvegicus]	96.7	96.7	33%	1e-21	65%
gi|254939230|CBA11532.1	linker for activation of T-cells family member 1 [Homo sapiens]	77.4	77.4	22%	2e-15	97%
gi|119572410|EAW52025.1	linker for activation of T cells, isoform CRA_b [Homo sapiens]	62.0	62.0	10%	1e-09	100%
gi|148685398|EDL17345.1	linker for activation of T cells, isoform CRA_a [Mus musculus]	38.5	38.5	9%	0.21	65%
gi|149067938|EDM17490.1	linker for activation of T cells, isoform CRA_b [Rattus norvegicus]	37.7	37.7	9%	0.38	62%

 

Phylogenetic tree:

Both the graphical representation of the BLAST search and the phylogenetic tree show that the Homo sapiens isoform a sequence is closely related to rodent and primate sequences, but not so closely related to carnivores. The search showed that there is a high degree of similarity between each of the sequences highlighted in red that is not due to chance. This is proven by the low E values. The close relation between different organisms indicates evolutionary conservation and means the amino acids interact with similar molecules. Hence, this means there could be a common signalling pathway and function of LAT across many organisms.